Investigation on the Removal of 2,4-chlorophenol Using Horseradish Peroxidase Immobilized on Nanohybrid

Abstract

Herein, in this report, was developed a nano-hybrid of Fe₃O₄/Ag/GO nanoparticles (NPs), constituted of graphene oxide (GO) supporting flower-like Fe₃O₄/Ag NPs in the presence of citric acid surfactant. The citric acid-functionalized Fe₃O₄/Ag/GO nanoparticles were used for Horseradish peroxidase (HRP) immobilization. The immobilized HRP can be tested to remove 2,4-dichlorophenols in the presence of H₂O₂, the degradation of 2,4-DCP at pH 6 at 25 °C and in the presence of H₂O₂ (0.8 mM) was for free and immobilized HRP of 40 % and 77 %, respectively. Moreover, immobilized HRP showed a high removal efficiency of ~94% in the presence of 0.6 mM of H₂O_2.<