Abstract
Because of its good stability, immobilized enzyme biosensor has become one of the main methods to prepare enzyme electrode, which is widely used in biosensors. The sensor cannot be used online due to the high temperature sterilization process. It cannot control the amount of glucose in real time, which affects the quality and yield of fermentation. For this reason, some scholars put forward the idea of using enzyme instead of immobilized enzyme membrane. The glucose biosensor of enzyme injection has reached the standard of practical application.
However, there is a slight difference in the detection time, stability and other performance indicators compared with the mature enzyme membrane sensor. In this paper, a new algorithm is designed to calculate the reaction constant and concentration of enzyme injection glucose biosensor based on the kinetic equation of enzyme reaction.
The accuracy of the algorithm is verified by experiments. The main content of this study is to detect the glucose solution of 1mg/ml and 2mg/ml by enzyme injection sensor. By recording the voltage versus time curve, the linear slope of the curve is obtained. The Michaelis constant Km of the enzyme involved in the reaction was calculated to be 1.59. The change of voltage gradient of 3mg/ml glucose standard solution can be obtained. In addition, the algorithm is incorporated into the concentration equation. The concentration of the measured object is 3.04mg/ml. The accuracy of the algorithm is 98.67%.
