Abstract
Lipase (E.C.3.1.1.3) is a versatile and key enzyme in various bioprocesses involving the esterification and transesterification reactions for biodiesel generation. However, the production and recovery of enzymes is very expensive and often constitute an obstacle to wide use in bioprocesses. In this context, the solid state fermentation (SSF) could be an low-cost alternative, since it allows the use of agro-industrial wastes with low added value and promoting the production of more concentrated biocatalyst. The utilisation these residues in the fermentations, not only minimize the quantity off these residues in the environmental, but also add value to raw material, trough the production of economical interesting substances. The objective of this work is to verify the lipase production using pretreated sugarcane bagasse as substrate inoculated with lipolytic microorganism. The sugarcane bagasse is an agro industrial residue with high availability in Brazil and can have multiple uses, representing 25 % to 30 % of the total weight of the sugar cane. The microorganisms used were Penicillium sp. and Rhizomucor sp. combined with temperature operating conditions of 28 °C, 33 °C and 38 °C, moisture content of 60 %, 70 % and 80 % and olive oil concentration 5 %, 7.5 % and 10 % as inducer. The study was performed by using factorial design of type (33) with central points. The results shown that best microorganism for lipase production was the Rhizomucor sp. (0.58 IU/gsubstrate), although there is very slight difference when compared to the Penicillium sp. (0.47 IU/gsubstrate). These results were found in the conditions of 33 °C, 80 % moisture content and 10 % of inducer for both microorganisms. After analyses statistic was verified that the moisture content of the medium interfered in the enzyme production for Penicillium sp. For the Rhizomucor sp. the moisture content and the concentration of the inductor olive oil interfered in the enzyme production.
