Abstract
With the development of society, competitive sports attracted a lot of attention. Coaches and athletes not only intend to improve the level of training, but also get the best performance by improving the auxiliary means of athletic ability, especially by applying some substances that contain no prohibited substances to enhance physical fitness, resist fatigue and give full play to the potential of physical exercise. In this paper, mitochondrial enzyme activity is investigated through 50 healthy SD male rats. After exercising, different groups of rats were killed one by one, decapitated, blood, then quickly skinned legs, remove thegastrocnemius, extracted mitochondrial were detected skeletal muscle mitochondrial Ca2+-ATP enzyme andH+-ATP activity changes, as well as indicators of muscle tissue superoxide dismutase (SOD) and malondialdehyde (MDA) to analyze the effect of fatigue which can guide the training of athletes.