Abstract
The components of dandelion root were extracted by ultrasonic-assisted extraction method and its chemical compositions were analyzed by GC-MS. Based on the single experiment, the extraction yield of components in dandelion root as the response value, the best condition of chemical components extraction was optimized by orthogonal array design and analysis. At last, the results showed that ultrasonic extraction time had the greatest impact on the extraction yield of chemical components, followed by the volume ratio of ethanol to ether, material-liquid ratio affected least. The optimum extraction conditions were the volume ratio of ethanol to ether 2:5, ultrasonic time 15 min, and material-liquid ratio 1:35 (g:mL), Under this condition, the average value of the experiment turned out to be 7.72%; Totally 17 compounds were identified by GC-MS, accounting for 89.97% of the total separated compounds. The major compounds identified were Germanicus(34.84%); Olean-18- en-28-olc acid, 3-oxo-, methyl ester(16.21%); 2H-1-Benzopyran-2-one, 6-acetyl-7 -(acetyloxy)-4- methyl-(14.14%); urs-20-en-3-ol(3ß,18a,19a)(11.49%); 6S-2,3,8,8- Tetramethyltricyclo [5,2,2,0](1,6)]undec-2-ene(2.15%); Genistein(1.51%); a-Amyrin (1.48%); urs-20-en -3-ol (3ß,18a,19a)(1.38%), ect.
