Abstract
Effects of various explants and hormone combinations on in vitro regeneration in cucumber (Cucumis sativus) were studied using Jinyou No. 1. The results showed that explant types had a greater impact on there generation rate and number of regenerated buds in cucumber. When using 1–2-d-old sterilized cotyledons as explants, Murashige and Skoog (MS) supplemented with 6-benzylaminopurine (6-BA; 3.0 mg L-1), kinetin (KT; 1.0 mg L-1), abscisic acid (ABA; 1.0 mg L-1), and AgNO3 (2.0 mg L-1) was the best bud induction medium, with an adventitious bud regeneration rate of 73.3%, and 1.00 regenerated bud per explant. When using 5–6- d-old sterilized cotyledonary nodes as explants, MS supplemented with 6-BA (2.0 mg L-1) and AgNO3 (2.0 mg L-1) was the best, with an adventitious bud regeneration rate of 90.70% and 4.38 regenerated buds perexplant. In this study, cotyledonary nodes were more suitable than cotyledons for the establishment of the in vitro regeneration system in cucumber, and MS containing 6-BA (2.0 mg L-1) was the best bud induction medium for cotyledonary nodes, which may have valuable applications for an efficient in vitro regeneration protocol.
