Xiong Y., Yang H., Zhu P., Huang H., He Q., Zhou J., 2015, Repaid and Accurate Detection of Khuskia oryzae by Loop- mediated isothermal Amplification Combined with a Lateral Flow Dipstick, Chemical Engineering Transactions, 46, 877-882.
Khuskia oryzae being as a contaminating fungus of jet fuel results in many damage. We have developed and valuated a repaid and accurate method for detection of Khuskia oryzae by loop- mediated isothermal amplification (LAMP) combined with a lateral flow dipstick (LFD).The result could be visualized by LFD within 10 min and did not rely on expensive experiment instrumentation. The detection limit of DNA of Khuskia oryzae by LAMP-LFD was 230fg/ul which was 10-fold more sensitive than LAMP and PCR methods.Khuskia oryzae could be specifically detected by LAMP-LFD, and no positive result could be observed when another 5 control genomic DNA were used. The whole process of detection by LAMP-LFD was 80 min which saved 90min compared to PCR method. The LAMP-LFD assay developed by this study was used for field detection and successfully detected Khuskia oryzae from the jet fuel samples. The LAMP combined with LFD makes the detection of Khuskia oryzae to be more simple, repaid and accurate, therefore, it will be a new tool for field detection.
