Valuation of The Seed Extract Moringa Oleifera for Removal of Cells Microcystis Sp. Enabling The Production of Biodiesel

Abstract

The objective of this study was to evaluate the lipid content in the sludge produced by the biomass of Microcystis protocystis, obtained in the process of water treatment coagulation/flocculation/dissolved air flotation (C/F/DAF), using as coagulant Moringa oleifera Lam (MO) seeds. For the tests was used deionized water artificially contaminated with cell cultures M. protocystis, obtaining a cell density of 10⁶ cells.mL^-1. Cyanobacterium cells were grown in ASM-1 liquid medium sparged with sterile air and maintained under conditions of maximum asepsis, controlled temperature around 24 °C under fluorescent lamps, with a 12h light-12 h dark photo period. The C/F/DAF process was carried out with a solution of 1 % moringa seeds at concentrations varied in the range of 25 - 300 mg.L^-1. Biomass lipid was evaluated by lipid composition of esters in transesterification/etherification reaction, adding to the oil, a solution of hexane, chloroform and hydrochloric acid. The esters produced were diluted in a solution of hexane and tricosanoic acid methyl ester as the internal standard in gas chromatographic analysis. Lipid material was characterized as the profile of the major fatty acid. Biomass showed a composition of palmitic acid (23.67 %), oleic acid (24.08 %) and linoleic (12.52 %). These results demostrated that cyanobacterium lipids from M. protocystis have interesting properties for biodiesel production.