Dias P., Ramos K., Padilha I., Araujo D., Santos S., Silva F., 2014, Optimization of Cellulase Production by Bacillus Sp. Isolated from Sugarcane Cultivated Soil, Chemical Engineering Transactions, 38, 277-282.
Cellulases are glycoside hydrolases involved in the depolymerization of cellulosic materials and have wide industrial applications, such as in the food, textile and feed industry. In addition, the animal feed industry uses cellulases to improve the digestibility of plant products. A technological application currently under study is the production of second-generation bioethanol, as a result of the search for alternative energy sources. The objective of this work was to use bacteria isolated from tropical soil used by the sugar and alcohol industry in the State of Paraíba (Brazil). These bacteria are Bacillus sp C1AC55.07 selected as producers of cellulolytic enzymes for the production of cellulases. The factorial planning methodology and the response surface analysis were used to evaluate the effect of cultivation temperature, yeast extract concentration and inoculum concentration on carboxymethylcellulase production. The experimental design used has three factors (2³), resulting in 11 experiments, since three of them were central point replications. The cultures were grown in 500mL Erlenmeyer flask containing 250mL of culture medium. Samples were collected at regular time intervals for analysis of the carboxymethylcellulase activity. The results showed that the highest carboxymethylcellulase activity (366 U / L) was obtained in 2 g / L yeast extract, 30% inoculum (v/v), and temperature of 32°C for 54 hours. Bacillus sp C1AC55.07 showed good cellulase activity and may be used in industrial applications.
