Mihos F., Silva L., Salgado A., Maggesissi M., 2014, Application and Construction of a Biosensor Using Graphite Rod and Bean, Phaseolus Vulgaris L., for Phenol Detection, Chemical Engineering Transactions, 38, 439-444.
This paper presents the possibility of fabrication and assembly of an amperometric biosensor for the phenol detection. The sensor was designed by immobilizing the bean powder containing peroxidase enzyme, with graphite rod as a conductor. Using the peroxidase activity, phenol can be oxidation in presence of hydrogen peroxide. The performance of the biosensor to phenol detection was based on the electron transfer detected by cyclic voltammetry. The proposed biosensor has a very sensitive response to phenol compounds, an applied potential of -0.2 to +0.8 mV vs Ag/AgCl. The manufacturing process parameters were optimized for the electrode. Experimental conditions that influenced the biosensor performance, such as pH, were investigated and evaluated. The response time of the biosensor was about 10 seconds.
