Fischer J., Lopes V., Santos E., Coutinho Filho U., Cardoso V., 2014, Second Generation Ethanol Production Using Crude Enzyme Complex Produced by Fungi Collected in Brazilian Cerrado (Brazilian Savanna), Chemical Engineering Transactions, 38, 487-492.
Bioconversion of agro-industrial residues has gained much focus worldwide as effective strategy to reduce environmental pollution and improve ethanol production. Therefore, the Brazil as one of the world's biggest agro-industrial residues producer can use residues such as rice straw, sugarcane bagasse and others to produce cellulolytic enzymes for enzymatic hydrolysis for second-generation bioethanol production. The objective of this work was to produce cellulolytic enzymes and use these enzymes to produce second generation ethanol from sugarcane bagasse. Twelve cellulolytic enzymes micro-organism from Brazilian Cerrado (Savanna) were tested to produce crude enzyme extract by solid state fermentation and the enzyme complexes were used to produce ethanol from exploded sugarcane bagasse using Saccharomyces cerevisiae Y904. All crude enzymatic complex used were able to produce ethanol from sugarcane bagasse, thus suggesting that the Brazilian Cerrado appears to be a promising source of cellulolytic enzymes. The best results were found for 48h of fermentation using crude enzymatic extract produced by Aspergillus niger ( 11.5 g/L of ethanol) and Mucor racemosus Fresenius (7.2 g/L of ethanol).
