MoS2/ZnO nanoparticles (NPs) were synthesized by a one step, highly promising synthesis approach, to be tested for the effective removal of hydrogen sulfide from drilling mud. The syntheses were performed by thermolysis of ammonium thiomolybdates (NH4)2MoS4 and zinc(II)acetylacetonate in a three necked flask under a flow of nitrogen. Zinc oxide NPs alone were also prepared. The samples were characterized by Raman Spectroscopy, Transmission (TEM-EDS) Electron Microscopy and X-ray diffraction (XRD). The synthesized NPs were used to remove hydrogen sulfide from water based drilling fluid. The efficiency of these NPs in the removal of hydrogen sulfide from drilling mud was evaluated and compared with that of ZnO NPs alone. The obtained results show that an amount of 3 g of MoS2/ZnO NPs is able to remove 100% of H2S (800 ppm) from water based drilling mud in just few minutes. On the other hand, 60 minutes are required in the case of 3 g of ZnO NPs alone.