Immobilization of Horseradish Peroxidase on Fe3o4/au_go Nanoparticles to Remove 4-chlorophenols from Waste Water

Abstract

Here we report the removal of 4-chlorophenols from waste water through the use of horseradish peroxidase (HRP) immobilized on Fe₃O₄/Au_GO nanoparticles. The large amount of surface functional groups on the support, which is coated with citric acid molecules, enables rapid enzyme immobilization through electrostatic interactions. HRP have been immobilized through physical adsorption on Fe₃O₄/Au_GO for 4-chlorophenols removal. The 4-chlorophenol degradation in presence of HRP immobilized on Fe₃O₄/Au_GO was 98 % after 180 min and at room temperature, with a reusability after three cycle of 95 %.