Prinz A., Zeiner T., Voessing T., Schuettmann I., Zorn H., Gorak A., 2012, Experimental Investigation of Laccase Purification Using Aqueous Two-Phase Extraction, Chemical Engineering Transactions, 27, 349-354.
In this paper the partitioning behavior of laccase from a culture supernatant of a Pleurotus sapidus fermentation in an aqueous two-phase system (ATPS) of polyethylene glycol with a molecular weight of 3000 g/mol (PEG 3000), potassium and sodium phosphate and sodium chloride is experimentally investigated. Results show that enzyme activity is retained during aqueous two-phase extraction. In addition the enzyme activity partitioning strongly depends on the sodium chloride concentration. The partitioning of impurities is not influenced with varying amounts of sodium chloride.
