This paper further develops a methodology for Regional Total Site Integration that is an extension of the Total Site Integration scope to the regional level. A previously developed general synthesis model by the authors has now been extended in this contribution for the purpose of Regional Total Site Integration applied within a demonstration case study of a regional biomass energy network. A multi-objective optimisation is performed as part of the synthesis for the evaluation of the maximal energy savings and footprint reduction within regional energy networks by accounting for the variability of demand and supply. The integration of renewable energy sources by maximising heat recovery within regional Total Sites clearly offers a significant contribution to regional energy savings, economic benefits, and footprint reduction.