Mycotoxins contamination and the associated risks are well known from the scientific community as it is possible to desume from the huge amount of the related scientific publications. Although the threat for human and animal health is really high and the worldwide diffusion of the contamination is for the most part uncontrollable, the public opinion is not really conscious of the problem and sometimes ignores it. In the present paper, a brief description of different aspects related to mycotoxins contamination is reported as regard toxicology and risks for the health, recent European and global regulations, and laboratory methods for mycotoxins determination.