Glucose sensors detection is a common method for glucose detection. Organic electrochemical transistors (OECTs) are considered to highly sensitive glucose sensors. The sensors show linear range from 0.1 µM to 100 µM, and its detection limit is about 0.1 µM. In addition, its sensing mechanism is discussed in detail. Glucose detection results show that the devices are sensitive to the gate voltage changes caused by the glucose enzymatic reaction. A glucose sensor detection system based on high-performance OECT-based glucose sensors is proposed. It is expected that the OECT devices have potential application on the low-cost and disposable glucose detection.