Abstract
Phenolics are part of a large group of pollutants, widely expanded throughout the medical, food and environmental matrices. They are main contaminants in ground and surface water. In view of their toxicity and persistence in the environment, the identification of phenolic compounds becomes an important matter. Enzymatic biosensors represent potential options to detect these species especially in case of in situ techniques. Particularly, biosensors based on polyphenol oxidase as well as tyrosinase or laccase have been developed for the determination of phenol derivatives. Due to the fact, here is reported a ceramic-based biosensing system fabricated through the immobilization of laccase in an electrochemically synthesized polymer - poly[2,7-bis(carbazole)-N-hexylacridone], based on low temperature co-fired ceramics technology (LTCC). This setup can be adequate for the permanent monitoring of aqueous solutions (i.e. presence of phenolics). The enzyme was immobilized on the surface of polymer film covering the microreaction chamber. The work of the biosensing system was estimated in the presence of ABTS (ammonium salt of 2,2'-azino- bis(3-ethylbenzthiazoline-6-sulphonic acid)), used as an enzymatic assay substrate. The setup revealed clear catalytic activity, and the analyte was optically determined. Due to the fact, the designed catalytic system showed sufficient efficiency, the obtained results suggest that, the phenoloxidase immobilization possesses potential to fabricate the bioelectronic devices.
