Synthesis of Macrocycle (MC) – Mimics the Properties of Natural Siderophores and Preparation the Nanostructures on the Basis of MC and Magnetite Nanoparticles

Abstract

In this work we report of the synthesis of hydroxyl containing azacrown macrocycle (MC) that is able to mimic the properties of natural siderophores. On the basis of synthesized MC were prepared the supramolecular ensembles with magnetite nanoparticles, loaded by cephalosporin antibiotics. The synthesized MC was investigated by NMR, mass-, FTIR spectroscopy methods. The morphology of prepared nano-ensembles was analyzed by scanning electron microscopy SEM, FTIR, X-ray diffraction XRD analysis methods. The quantitative analysis of nanostructures was determined by atom absorbance spectroscopy (AAS) as well as on the basis of Lambert-Beer law by UV spectroscopy method. Prepared nanostructures were tested on gram- negative Escherichia Coli and gram-positive Staphylococcus aureus, having multi drug resistance properties. Has been found that the nanostructures significantly increase the antimicrobial effect of cephalsporins and decrease their MIC.