Optimisation of Medium Composition and Culture Conditions of Filter-paperase by UPMC1106 using Pennisetum Purpureum

Abstract

Lignocellulosic resources are valuable and widely utilised to produce fermentable sugar. There is potential non-human consumption lignocellulosic material such as Pennisetum sp. available in the tropical and subtropical region. This study was conducted to optimise the medium composition and culture conditions of submerge fermentation of locally isolate UPMC1106, using Pennisetum sp. as the substrate in shake flasks. The Pennisetum sp. or Pennisetum purpureum sp. was collected from Pahang, Malaysia (3.793619° N, 102.523449° E) at the age of three months, processed until milled using Thomas Wiley mill and passed through a 60-mesh sieve. The powder form Pennisetum sp. was used as the sole carbon source in the cultivation medium. Four factors were chosen for the optimisation, as these factors contributing to the cellulosic enzymes production. The factors were Pennisetum sp. concentration, initial medium pH, agitation speed, and inoculum size. For the maximum filter-paperase activity, FPase, (U/mL), the Napier concentration was found to be 4.3 % (w/v), and the initial medium pH was 7.8. The optimum agitation speed and inoculum size were achieved at 170 rpm and 19.7 % (v/v). The optimum values of the respective factors gave 2.895 U/mL of FPase activity. This response was 98.8 % close to the predicted value and 30 % of improvement from the non-optimised factors.