Abstract
Microwave assisted aqueous enzymatic extraction (MAAEE) of Elateriospermum Tapos Seed (ETS) was performed in this study. This is a novel method which can reduce the extraction time and solvent consumption. The Elateriospermum Tapos oil (ETO) yield was optimised by using a central composite design (CCD) at condition parameters of microwave power (110 - 1,100 W), extraction time (30 - 120 s), enzyme cocktail concentration (1 - 5 %) and particle size (0.5 - 1.5 mm). The effect of each parameter was investigated by Response Surface Methodology (RSM). The optimal oil extraction yield was obtained at 110 W microwave power, 30 s extraction time, 1 % enzyme cocktail concentration and 0.5 mm particle size. Under these conditions, the maximum value obtained for the oil extraction yield was 46.12 ± 1.48 % recovery which was in good agreement with the predicted value (43.87 %). Thus, MAAEE could speed up extraction process as compared to Soxhlet which required 6 h extraction time to achieve similar amount of extraction yield.
