Abstract
The chemical molecular probe belongs to chemical sensors, and they mainly use fluorescence to convey the change of chemical information produced by analytic target and sensitive molecules. The transfer of this information has the advantages of high sensitivity and high selectivity, and only needs a little samples. It is widely used in chemistry analysis, especially in biological analysis. The labeling reagents with strong fluorescent or the reagents generated by fluorescent can be used for the object to be tested to make it have fluorescent labeling characteristic, which will greatly improve the detection sensitivity and reduce the detection limit. The data show that the sensitivity detected by this method can reach 10-9, and the lowest has reached 10-12, which is the fluorescent probe technology. It has many advantages such as high selectivity, high sensitivity, simple operation, high accuracy and simple equipment. In recent years, many researchers have paid attention to it. In this paper, the small molecule fluorescent probe with thiophene [2, 3-b] thiophthene as fluorophore is designed, because thiophthene is a large conjugated system, the electron cloud density is relatively high, and it has hole transport ability and good optical properties. We chose benzothiazole as the main recognition perssad. In the study, we also use the chloroacetyl chloride reagent to adjust the distance between luminophor and the recognition objects, and to increase the number of atoms with coordination of probe molecules, and hope to strengthen complexing power of them with metal ion. The results show that when the recognition object is near the luminophor, the molecular probe has significant double recognition effect on Zn2+ ion and Hg2+ ion, and when they are far away from each other, it only has the recognition effect on the Hg2+ ion.
