Abstract
As a kind of detection technology based on optical characteristics, spectral detection technology overcomes the defects of traditional detection technology. The common spectrum detection techniques include hyperspectral imaging, Raman spectroscopy and infrared spectroscopy. As a new detection technology, the detection efficiency is high and the time is short. It is easy to realize automation and is suitable for on-line detection of mass production. In the process of yeast culture, the content of glycerol and methanol were monitored in real time. The results show that the application of infrared spectrum detection technology and ATR probe can realize off-line and on-line monitoring of glycerol and methanol. The correlation coefficients of infrared prediction model were 98.65% and 98.09%, respectively. The results showed that it costed 40 hours from the culture stage to the beginning of the addition of glycerol. The time required for methanol consumption was 6 hours. The experimental results proved the possibility of the application of infrared detection technology in the industrial on-line monitoring.
