In this paper, the adsorption and anoxic degradation of No. 0 diesel oil in Bohai coastal sediments were studied. The results showed that the adsorption of oil on the coastal sediments matched pseudo-second-order kinetics, which indicated that adsorption processes were essentially chemical reactions. Part of oil could be degraded in natural coastal sedimentary environment, and the additions of nutrients and proper electron acceptors could promote the degradation of oil effectively. The addition of nitrogen and phosphorus nutrients increased the removal ratio of oil from 54 % to 61 %. Different electron acceptors showed different effects on the degradation of oil in coastal sediment. Nitrate greatly promoted the degradation of oil, but sulfate had an adverse effect on that. Three oil-degrading strains named B1, B2, B3 were domesticated and isolated from Bohai coastal sediments. All the three strains could promote the degradation of oil, and the effect of B2 on the degradation of oil was the most significant, which increased the removal ratio of oil by nearly 30 %.
