Ramos A., Espinosa A., Mendez D., Cabeza I., Moreno - Sarmiento N., 2018, Purification of Poly (3-hydroxybutyrate) Produced by Fatty Acid Fermentation Using Organic Polar Solvents, Chemical Engineering Transactions, 67, 667-672.
Poly (3-hydroxybutyrate) or (P3HB) produced by the fermentation of fatty acids with the mutant strain Burkholderia cepacia B27 was extracted and purified using different techniques including washing and precipitation of the biomass, and the polymeric materials with organic polar solvents such as ethanol (96%) or methanol (99,7%). The materials obtained after the purification processes were thermally characterized (using differential scanning calorimetry and thermo gravimetric analysis). The polymeric samples treated with organic polar solvents showed improved melting point passing from 169,2°C; for the chloroform purified samples to 174,9°C for the methanol purified samples, also, appearance and odor of the films was improved.