Abstract
The present study aims to develop an efficient industrial fermentation process that promotes the increase of sporulated biomass in a single batch of Bacillus coagulans BVB5. Preliminary studies of various culture media were performed in Erlenmeyer flasks for 96 hours in a shaker at 44 °C and 240 rpm, and the degree of sporulation was analyzed. The culture media tested were glucose, yeast extract, peptone based medium (GYP), GYP with salts (GYPs), glucose, yeast extract, tryptone based medium (GYT), soy protein, yeast extract, sodium chloride based medium (FNY), 50% FNY (FNY50), glucose, soy protein, potassium salts based medium (DSK) and 50% DSK (DSK50). The results had shown that the FNY50 medium obtained a higher degree of sporulation of 100% followed by FNY with 99.99%, whereas media containing relatively large amounts of glucose such as GYP, GYPs and GYT did not exhibit satisfactory sporulation. The reduction of the cost and consequent increase of the efficiency in the preparation of a probiotic microorganism of BVB5 in the DVS format are the main benefits of reducing the time of the fermentation/sporulation process. For this, more studies are ne
