Lipase from Thermomyces lanuginosus was physically anchored to AgAu-Fe3O4@tartaric acid nanoparticles (NPs) to be used for the conversion of levulinic acid into ethyl-levulinate, in the presence of ethanol. The proposed strategy explored the direct immobilization of the enzyme on the as-prepared tartaric acid modified NPs. A very remarkable conversion of 90 % was achieved at 4:1 ETOH/acid molar ratio, after 12 h and at 45°C reaction temperature, higher than that shown by free lipase.