Water Treatment from Phenol Derivatives by the Oxidoreductases Immobilized on Alginate Microspheres

Abstract

In this paper, the synthesis of novel effective biocatalysts based on oxidoreductases (peroxidase extracted from the horseradish roots; commercial glucose oxidase) immobilized on the sodium alginate microspheres was performed. To activate the carboxylic groups of the support, enzyme immobilization on the sodium alginate microspheres was carried out using carbodiimide and N-hydroxysuccinimide. This allows the successful crosslinking of enzyme and support to be reached. The investigation of catalytic properties of the synthesized biocatalysts was carried out in the oxidation of phenol and 4-chlorophenol in the presence of 4-amino antipyrine. The activity and stability of the synthesized biocatalysts was studied. The use of biocatalysts immobilized on the sodium alginate microspheres allows more than 95 % of phenol and 4-chlorophenol conversion to be achieved. The synthesized biocatalysts exhibit 25 - 30 % activity in comparison with the native enzyme in the oxidation of phenol and its derivatives. The biocatalysts were found to save their activity in the up to 10 multiple reuses.