Analysing the Influence of Cultivation Conditions on the Activity of Metabolic Pathways of Bcaa Biosynthesis in Chlorella Vulgaris Microalgae

Abstract

The influence of cultivation conditions on the productivity of strains of Chlorella vulgaris Beijer IPPAS C-1 (Chlorella sorokiniana), Chlorella vulgaris Beijer IPPAS C-2, Chlorella kessleri Fott et Nov C-9 (Parachlorella kessleri), Scotiellopsis Vinatzer IPPAS C-35, Chlorella vulgaris Beijer IPPAS C-38 has been studied experimentally. The microalgae strain Chlorella vulgaris Beijer IPPAS C-1 (Chlorella sorokiniana) was found to be promising in terms of protein production. Under autotrophic cultivation conditions at PAR = 150 µmol photons/m²•s and temperature 30 ± 2 °C the amount of the biomass has increased by 10-15 times during 4-5 days of cultivation. When the cultivation temperature is lowered under mixotrophic conditions, Chlorella vulgaris Beijer IPPAS C-1 increases the synthesis of intracellular proteins and branched-chain amino acids.