Modeling the Specific Glucose Consumption Rate for the Recombinant E.coli Bioprocesses Based on Aging-specific Growth Rate

Abstract

This study proposes the form of the physiological E.coli dependencies for the specific glucose consumption rate. It carries a two-fold purpose. The first one allows online estimation of the specific glucose consumption rate when the information on the cells' aging is accessible. The second relationship assesses that the glucose consumption is relative to the oxygen uptake rate content with no direct dependency on the biomass kinetics properties. Such portion of the oxygen uptake rate does not participate in biomass growth or maintenance-related state but instead increases the soluble by-product of metabolism. The latter can consist of extracellular metabolites, soluble proteins, or other by-products. The authors show that these solutes assumingly result in the corresponding boundary condition for the specific glucose consumption rate balance. The main novel idea of this work is the statement that the portion of oxygen uptake rate dedicated for soluble products does not depend on the biomass growth and maintenance but rather on average aging information. Moreover, the authors hypothesize that biomass yield from glucose can be treated as a function of the average aging information of the cell population by reusing the Monod term assumption. Finally, this study interconnects some of the co-authors' patent claims that directly link to the empirical law of the Luedeking-Piret.