Design Biocatalyst: the Influence of the Nature of the Carrier on the Catalytic Properties of Immobilized Glucose Oxidase

Abstract

Mesoporous silica, alumina and zirconia, were utilized as appropriate support for the formation of magnetite nanoparticles by in-situ crystallization and GOx immobilization. Magnetic and nonmagnetic supports were functionalized with amino groups and using glutaraldehyde as a linker. A comparative study showed that the nature of the carrier and the presence of magnetite nanoparticles all affect the catalytic properties of biocatalysts and their stability. Biocatalysts based on immobilized glucose oxidase demonstrated high catalytic activity (preservation of the activity of the free enzyme up to 98 %) and stability in D-glucose oxidation to D-gluconic acid at pH 4-8 and temperature of 30-60 °C.